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Implementation of a Multi-Institutional Diffuse Intrinsic Pontine Glioma Autopsy Protocol and Characterization of a Primary Cell Culture.


Posted on: 08/09/2012

Neuropathol Appl Neurobiol. 2012 Jul 27. doi: 10.1111/j.1365-2990.2012.01294.x. [Epub ahead of print]

Implementation of a Multi-Institutional Diffuse Intrinsic Pontine Glioma Autopsy Protocol and Characterization of a Primary Cell Culture.


Caretti V, Jansen MH, van Vuurden DG, Lagerweij T, Bugiani M, Horsman I, Wessels H, van der Valk P, Cloos J, Noske DP, Vandertop WP, Wesseling P, Wurdinger T, Hulleman E, Kaspers GJ.
Source
Department of Pediatric Oncology, Neurosurgical Center Amsterdam, Neuro-oncology Research Group, Department of Pathology, Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands, Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands, Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Abstract
AIMS:
  Diffuse intrinsic pontine glioma (DIPG) is a fatal paediatric malignancy. Tumour resection is not possible without serious morbidity and biopsies are rarely performed. The resulting lack of primary DIPG material has made preclinical research practically impossible and has hindered the development of new therapies for this disease. The aim of the current study was to address the lack of primary DIPG material and preclinical models by developing a multi-institutional autopsy protocol.

METHODS:
  An autopsy protocol was implemented in the Netherlands to obtain tumour material within a brief post-mortem interval. A team of neuropathologists and researchers was available at any time to perform the autopsy and process the material harvested. Whole brain autopsy was performed and primary DIPG material and healthy tissue were collected from all affected brain areas. Finally, the study included systematic evaluation by parents.

RESULTS:
  Five autopsies were performed. The mean time interval between death and time of autopsy was 3 hours (range 2-4). All tumours were graded as glioblastoma. None of the parents regretted their choice to participate, and they all derived comfort in donating tissue of their child in the hope to help future DIPG patients. In addition, we developed and characterized one of the first DIPG cell cultures from post-mortem material.

CONCLUSION:
  Here we show that obtaining post-mortem DIPG tumour tissue for research purposes is feasible with short delay, that the autopsy procedure is satisfying for participating parents and can be suitable for the development of preclinical DIPG models. © 2012 The Authors. Neuropathology and Applied Neurobiology © 2012 British Neuropathological Society.

© 2012 The Authors. Neuropathology and Applied Neurobiology © 2012 British Neuropathological Society.

PMID: 22845849 [PubMed - as supplied by publisher]
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